https://insight.jci.org/articles/view/136539
https://doi.org/10.1172/jci.insight.136539
https://pubmed.ncbi.nlm.nih.gov/32641584/
TITLE:
Linking epigenetic dysregulation, mitochondrial impairment, and metabolic dysfunction in SBMA motor neurons
ALTERNATIVE TITLE:
None
DATE:
Verify – Thu, 09 Jul 2020 06:00:00 -0400
AUTHORS:
Naemeh Pourshafie,Ester Masati,Eric Bunker,Alec R Nickolls,Parisorn Thepmankorn,Kory Johnson,Xia Feng,Tyler Ekins,Christopher Grunseich,Kenneth H Fischbeck
SOURCE:
JCI insight
DESCRIPTION:
Spinal and bulbar muscular atrophy (SBMA) is a neuromuscular disorder caused by a polyglutamine expansion in the androgen receptor (AR). Using gene expression analysis and ChIP sequencing, we mapped transcriptional changes in genetically engineered patient stem cell-derived motor neurons. We found that transcriptional dysregulation in SBMA can occur through AR-mediated histone modification. We detected reduced histone acetylation, along with decreased expression of genes encoding compensatory…
CONTENT:
Pourshafie N, et al. JCI Insight 2020.
ABSTRACT
Spinal and bulbar muscular atrophy (SBMA) is a neuromuscular disorder caused by a polyglutamine expansion in the androgen receptor (AR). Using gene expression analysis and ChIP sequencing, we mapped transcriptional changes in genetically engineered patient stem cell-derived motor neurons. We found that transcriptional dysregulation in SBMA can occur through AR-mediated histone modification. We detected reduced histone acetylation, along with decreased expression of genes encoding compensatory metabolic proteins and reduced substrate availability for mitochondrial function. Furthermore, we found that pyruvate supplementation corrected this deficiency and improved mitochondrial function and SBMA motor neuron viability. We propose that epigenetic dysregulation of metabolic genes contributes to reduced mitochondrial ATP production. Our results show a molecular link between altered epigenetic regulation and mitochondrial metabolism that contributes to neurodegeneration.
PMID:32641584 | DOI:10.1172/jci.insight.136539
PUBMED ID:
pubmed:32641584
OTHER ID:
pmid:32641584,doi:10.1172/jci.insight.136539
PUBLICATION DATE:
Thu, 09 Jul 2020 06:00:00 -0400
2020-07-10
RETRIEVAL DATE :
07/09/20 07:06AM
LINK – PUBMED:
https://pubmed.ncbi.nlm.nih.gov/32641584/?utm_source=MS-Office&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1l7lJQBQXfTBJkL86rnvYKzafMiKbgcUrlv_X8_D_H5EuRmkjR&fc=20200708141943&ff=20200709070639&v=2.10.1
LINK – DOI:
https://doi.org/10.1172/jci.insight.136539
LINK – FULL TEXT:
Pending
NOTES:
None